1.
Protective Effect Of Diltiazem Hydrochloride On Occurrence Of Alloxan Induced Diabetes In Rabbits
by Azeem, M | Dr.Muhammad Razzaq Ali | Dr.Haji Ahmad | Dr.Muhammad Sabir.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 1998Dissertation note: An investigation was carried out to study the protective effect of calcium channel blocker i.e. diltiazem hydrochloride on occurrence of alloxan diabetes in rabbits and also to see the protective effect of a herbal mixture of medicines, i.e. Kalonji, Kasni and Maithee on occurrence of alloxan induced diabetes in rabbits.
For this purpose, 20 rabbits were randomly divided into four groups i.e. A, B, C and D, comprising 5 rabbits in each and were provided food and water libitum.
Each of the members of group A (control) were given 150 mg/kg body weight alloxan intravenously into ear vein. These animals were given food and water libitum for two days, then they were fasted over night and blood samples were collected for estimation of glucose and total protein.
The results obtained showed significant increase in blood glucose level subsequent to the administration of alloxan. There was no change observed in total protein.
The rabbits of group B were given 20 mg/kg body weight of Diltiazem hydrochloride intraperitoneally and after 15 minutes alloxan was administered intravenously. The results obtained showed significant increase in blood glucose level and no effect on total protein.
Similarly the rabbits of group 'C' were given 40 mg/kg body weight of diltiazem hydrochloride intraperitoneally and then after 15 minutes 150 mg/kg body weight of alloxan was administered intravenously into ear vein. Blood samples showed significant decrease in blood glucose level and no effect on total protein.
While rabbits of group-D were given 2 gm/kg body weight of herbal mixture orally, and then after two hours alloxan was administered intravenously. The data showed a significant rise in blood glucose level and again no effect on total protein. This showed that the herbal mixture had no protective effect.
From this result, it is conceivable that diltiazem hydrochloride showed protective effect on occurrence of alloxan induced diabetes in rabbits and there was no effect on total protein whereas the herbal mixture did not have protective effect.
Availability: Items available for loan: UVAS Library [Call number: 0577,T] (1).
2.
Synthesis And Evaluation Of Biological Properties Of Derivatives Of Cephalosporins
by Rehmat Ullah, Major | Dr.Muhammad Sabir | Dr.Haji Ahmad | Dr.Muhammad Raziq Ali.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 1999Dissertation note: This study was aimed at preparation and evaluation of the biological properties of cephradine. Schiff base transition metal complexes of cephradine were prepared. In the first step Schiff bases were prepared by condensation of the primary amine with a carbonyl compound. For this purpose pyridoxal HCI and salicylaldehyde were used. In the next step the prepared Schiff bases were chelated with metal salts using copper acetate and zinc acetate, here metal binds to polydenate ligands to form the ring structure, where metal is the part of the ring. In this way a three dimensional molecule was obtained which exhibited enhanced antibacterial activity in some cases and depressed activity in the others. The complexes were subjected to biological evaluation to find out the minimum lethal concentration of the new complexes and then it was compared with the parent drug. The standard organisms of S. aureus, .E.coli Pseudomonas aeruginosa and . pneumoniae were used to study the antimicrobial activity. Cephradine complexes showed a depressed activity against S. aureus, . E.coli and P . aemginosa. But the activity of CPC and CSC derivatives was found ten times enhanced against
K. pneumoniae, when compared with the present drug. The haematological study revealed that the parent drug and the new derivatives did not have any deleterious effect upon the total erythrocyte count, haemoglobin concentration, leukocytic count, differential leukocytic count i.e., lymphocytes and polyrnorphs (neutrophils). Thus the new compounds were found to be as safe as the parent drug itself.
Availability: Items available for loan: UVAS Library [Call number: 0602,T] (1).
3.
Synthesis And Evaluation Of Bioilogical Properties Of Derivatives Of Ceftriaxone Sodium
by Shahid Zaigham | Dr.Muhammad Sabir | Dr.Muhammad Razaq Ali.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 1999Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 0622,T] (1).
4.
Study Of Changes In Hematological Laboratory Profile Induced By Vinorelbine In Cancer Patients
by Taha Nazir | Prof.Dr.Saghir Ahmad Jafri | Dr.Muhammad Mehmood Khan | Prof.Dr.Muham.
Material type: ; Format:
print
Publisher: 2000Dissertation note: The present study was carried to investigate the hematological alterations in the patients diagnosed as cancer and were administered vinorelbine as part of their chemotherapy at Shoukat khanum memorial cancer hospital and research centre. It was carried in the chemotherapy department, pathology laboratory, medical record section, pharmacy department, and data processing department of the hospital. For investigating there were a total of 60 adult cancer patients with non-small cell lung cancer (NSCL), metastatic breast cancer (MBC), and cancer of cervix (CC), with age between 24 to 71 years (Mean±SEM: 42.73±2.69) selected. Which were divided into two groups: Group - I for those patients who were on the treatment protocol of Vinorelbine alone (25 mg/m2/day one, weekly x 4) and group - II for those patients who were on treatment protocol of Vinorelbine base combinations Vinorelbine/Doxorubicin (Vinorelbine - 20 mg/m2/day one and day eight, Doxorubicin - 50 mg/m2/day one only) and Vinorelbine/Cisplatin (Vinorelbine - 20 mg/m2/day one and day eight, Cisplatin - 40 mg/m2/day one only). The Laboratory tests (C.B.C.) of all the patients were performed in the Pathology Laboratory of the hospital. After completion of the study, the collected Hematological values were statistically analyzed by student pair test. By the overall comparison of mean values over time, there were significant leukopenia (neutrophile, eosinophil, & monocyte), anemia and decreased hematocrit value observed in the patients on chemotherapy protocol of Vinorelbine alone and significant leukopenia (neutrophile only), anemia and decreased hematocrit value and hemoglobine conc. observed in the patients on chemotherapy protocol of Vinorelbine based combinations. By an independent comparison of mean values of two groups at every week, there were significantly higher toxicities 1 e leukopenia at week-3 (neutrohpil at week-3, eosinophil at weekI, lymphocyte at week-i &2) and thrombocytopenia at week-i observed in the patients on the treatment protocol of Vinorelbine based combinations. When the mean values observed before therapy were compared with that of at week-4 of both of the groups, there were significant decreases noted in TLC, neutrophil count, monocyte count, erythrocyte count, hemotocrit value and Hb conc in the patients on treatment protocol of Vinorelbine alone, and erythrocyte count, hemotocrit value and hemoglobine conc. in the patients on treatment protocol of Vinorelbine based combinations. It is concluded from this study that, there is insignificant difference in the overall hematological toxicities produced by both of the chemotherapy protocols. The clinical oncologist, consultant physicians and pharmacist, therefore now suggested to select the either of the of the chemotherapy protocol with respect of the therapeutic efficacy only
Availability: Items available for loan: UVAS Library [Call number: 0661,T] (1).
5.
Efficacy Of A Newly Developed Herbal Diuretic Mixture In Broiler Chickens
by Mahreen Zahara | Prof.Dr.Muhammad Ashraf | Dr.Muhammad | Dr.Muhammad Ovais Omer.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 2002Dissertation note: A number of diuretic drugs are presently used in poultry under the name of "flushers". These drugs play an important role in poultry disorders especially in cases of ascties, liver disfunctions, nephritis, Urates deposition, restoring normal functions of kidneys by eliminating toxic substances and extra fluid (through urinary system) and in oedematous conditions. The present study was under taken to evaluate the efficacy of a new locally prepared herbal diuretic mixture containing Cuminum Cyminum, Robonate of Poast, Sneeze wort and Salt petre and was compared with uronex in broiler chicken.
For this purpose, one hundred a day-old boiler chicks were reared under standard conditions. At the age of 28 day they were randomly divided into groups A, B, C, D and E. The birds of group A, B and C were given herbal diuretic mixture at different dilutions 1%, 2% and 3% in drinking water. The birds of group D were given uronex in drinking water and birds of group E were given with normal water, being control group. Diuretic efficiency of this herbal mixture was evaluated by measuring total serum protein, electrolytes (sodium and potassium) creatinine and uric acid contents before and after drug administration in broiler chicks.
Non significant (P>O.05) changes were observed in the serum protein and serum creatinine levels. The values of serum sodium, potassium and uric acid were significantly (P< 0.05) decreased in treated birds after medication as compared to control birds.
From these observations it is concluded that the newly prepared herbal diuretic mixture was effective and can be successfully used for the elimination of toxic and oedematous fluids in broiler chicks.
Availability: Items available for loan: UVAS Library [Call number: 0813,T] (1).
6.
Role Of Alpha-2-Adrenocepter Agonists As Sedative And Analgesic In Fancy Birds
by Uzma Farid Durrani | Prof.Dr.Muhammad Asharaf | Dr.Muhammad | Dr.Muhammad Ovais Omer.
Material type: ; Format:
print
Publisher: 2003Dissertation note: This project was designed to study the role of alpha-2-adrenocepter agonists as sedative and analgesic in fancy birds. A study was conducted on budgerigars pigeons and quails which were devided into three groups i.e. group A (budgerigar), group B (pigeon) and group C (quail), each group with fifty birds. Each group was subdivided into five subgroups i.e. X, D, K, X-K and D-K, each subgroup with 10 birds. All birds were physically examined and weighed. In group A, all birds of subgroups X, D, K, X-K, D-K were given xylazine (20mg/kg), detomidine (2.8mg/kg), ketamine (80mg/kg), xylazine+ketamine (1 Omg/kg+4Omg/kg) detomidine+ketamine (1 .4mg/kg+4Omg/kg) respectively via intramuscular route. In group B, all birds of subgroups X, D, K, X-K, D-K were given xylazine (16mg/kg), detomidine (1 .4mg/kg), ketamine (60mg/kg), xylazine+ketamine (8mg/kg+3 0mg/kg), detomidine+ketamine (0.7mg/kg + 30mg/kg) respectively via intramuscular route. In group C, all birds of subgroups X, D, K, X-K, D-K were given xylazine (18mg/kg), detomidine (2.4mg/kg), ketamine (60mg/kg), xylazine+ketamine (9mglkg+30mg/kg), detomidine+ketamine (1 .2mg/kg+3 0mg/kg) respectively via intramuscular route.
Different parameters i.e. time and nature of induction, duration, recovery, temperature, respiration, heart rate, reflexes and degree of analgesia were used for the evaluation of sedation and anaesthesia.
The results of this study indicated that in budgerigars, for non surgical procedures, xylazine (alone) can be used safely. For minor and major surgical procedures of budgerigars xylazine-ketamine combination can be used safely. In pigeons, for non surgical procedures, detomidine (alone) can be used safely; however, in case of unavailability of detomidine second choice is xylazine (alone). For minor and major surgical procedures of pigeons either xylazine-ketamine combination or detomidine-ketamine combination can be used safely. In quails, for non surgical procedures, xylazine (alone) can be used safely while for minor and major surgical procedures of quails, xylazine-ketamine combination can be used safely. Second choice for this purpose is detomidine-ketamine combination. All sedatives and anaesthetics should be used with proper thermoregulatory measures.
Availability: Items available for loan: UVAS Library [Call number: 0835,T] (1).
7.
Effect Of Levamisole On The Cholinesterase Inhibition By Trichlorfon In Rabbits
by Hafiz Muhammad Irfan | Dr.Muhammad Ovais Omer | Prof. Dr. Muhammad Ashraf.
Material type: ; Format:
print
Publisher: 2007Dissertation note: This project was designed in female rabbits to find out Trichiorfon- induced toxicosis and its interaction with Levamisole by inhibiting cholinesterase enzyme, taking account of the administration of levamisole before trichiorfon dosing. For this purpose, twenty female rabbits with weights of 1.0kg to 1.9kg were divided into four groups. Each group (A, B, C and D) containing five rabbits. Group 'A' was considered as control, group B was given trichiorfon 10mg/kg body weight (orally), while group 'C' was treated with levamisolelomg/kg (S/C) and then trichiorfon 10mg/kg (orally) after 30minutes of levamisole and the group 'D' was given levamisole 10mg/kg (S/C). After determination of butyryl cholinesterase activity, alkaline phosphatase activity, aspartate aminotransferase activity and alanine aminotransferase activity, it was observed that Trichiorfon and Levamisole significantly inhibited butyryl cholinesterase enzyme at 10mg/kg body weight Where as Levamisole pretreatment did not potentiate the inhibitory activity of Trichlorfon at 10mg/kg body weight dosage whether the Levamisole was given subcutaneously or orally. The alkaline phosphatase activity was increased significantly with Trichiorfon and Levamisole had no significant effect on it while the effect on aspartate aminotransferase was non significant. The alanine aminotransferase activity was decreased significantly with Levamisole. The results also showed that the time interval between Levamisole and Trichiorfon dosage and route of administration did not affect the cholinesterase activity. No clinical signs and postmortem lesions were observed at 10mg/kg body weight dosage while Levamisole at 50mg/kg body weight, produced signs of toxicosis. In general, there was no adverse drug interaction between Levamisole and Trichlorfon.
Availability: Items available for loan: UVAS Library [Call number: 0975,T] (1).
8.
Tha Anti-Inflammatory And Antipyretic Activity Of Nigella Sativa (Black Seeds, Kalonji) On Induced Inflammation And pyrexia in rabbits
by Muhammad Atif Raza Cheema | Prof.Dr.Muhammad Ashraf | Dr.Muhammad | Dr.Muhammad Ovais Omer.
Material type: ; Format:
print
; Nature of contents: ; Literary form: Publisher: 2007Dissertation note: Scientific interest in medicinal plants has grown rapidly due to increased efficiency of new plant-derived drugs, growing interest in natural products, and rising concerns about the side effects of conventional medicine. Before being considered for clinical trials in humans, the active ingredients of these plants should be identified and must show tolerable levels of toxicity in several animal models. Today, there are at least 120 distinct chemical substances derived from plants that are considered as important drugs currently in use in one or more countries in the world. More than 150 studies conducted since 1959 confirmed the pharmacological effectiveness of N. sativa seed constituents. N. sativa seed is a complex substance of more than 100 compounds, some of which have not yet been identified or studied. A combination of fatty acids, volatile oils and trace elements are believed to contribute to its effectiveness. The seeds of Nigella sativa Linn. (Ranunculaceae), commonly known as black seed or black cumin, are used in folk (herbal) medicine all over the world for the treatment and prevention of a number of disease conditions such as asthma, diarrhoea and dyslipidaemia etc. Much of the biological activity of the seeds has been shown to be due to thymoquinone, the major component of the essential oil, but which is also present in the fixed oil. The plant's mechanism of action is still largely unknown. The present study was designed to study the anti-inflammatory and antipyretic effect of Nigella Sativa oil on mammals (Rabbits). Another hypothesis is that Nigella Sativa oil has no hepatotoxicity in treated rabbits was also tested in this study. It has been observed that Nigella Sativa oil has anti-inflammatory and antipyretic activity along with no hepatotoxicity in treated rabbits. The biochemical analysis including ALT and AST showed variations from normal range after injecting LPS and turpentine oil. However, after administering LPS and turpentine oil along with Nigella sativa oil showed normal range of ALT and AST values as compared to values of the control group. These findings indicate that Nigella sativa oil may have protective action against hepatotoxicity in rabbits. All the results showed that Nigella sativa oil can also be used as an anti-inflammatory and antipyretic agent in veterinary practice is less toxic to aspirin. This study therefore, supports its use in folk medicine both as antipyretic and anti-inflammatory agent and calls for further investigations to elucidate its mechanism of action.
Availability: Items available for loan: UVAS Library [Call number: 0991,T] (1).
9.
Efficacy Of Florfenicol Against Haemorrhagic Septic Aemia In Bufalo Calves
by Shoaib Ashraf | Dr.Muhammad Ovais Omer | D. /Sjeruar Afzal | Prof. Dr. Azhar Maqboo.
Material type: ; Format:
print
Publisher: 2008Dissertation note: Two antibiotic preparations of florfenicol and amoxicillin were used in these trials against haemorrhagic septicaemia, singly and along with combination of a non-steroidal-anti-inflammatory-drug (flunixin meglumin).
Forty buffalo calves suffering from haemorrhagic septicaemia were selected and treated under field conditions. Weighed clinical score was recorded before and after treatment in each case on the basis of severity of clinical symptoms. Reduction in this score and recovery or death of animal was also noted.
It was concluded that florfenicol was more effective than amoxicillin and the
n of non-steroidal-anti-inflammatory-drug reduced convalescence period and also jincreased the survival rate. Therefore florfenicol along with flunixin meglumin is ecommended as the successful therapy for haemorrhagic Septicaemia.
Availability: Items available for loan: UVAS Library [Call number: 1004,T] (1).
10.
Comparative Therapeutic Efficacy Of Herbal Medicine(Cocciguard) With Standard Anticoccidial Drugs In Experimentally induced Coccidiosis in Broiler Chicks
by Khalid Khan | Dr.Muhammad Ovais Omer | Prof.Dr.Azhar | Prof.Dr.Muhammad Ashraf.
Material type: ; Format:
print
Publisher: 2008Dissertation note: This study was designed to compare and evaluate therapeutic anticoccidial efficacy of herbal drug (steroidal sapogenin), suipha drug (suiphachiorpyrazine) and Amproliurn.
Total of 150, a day-old broiler chicks were reared for six weeks in good husbandry and hygienic conditions. Coccidiosis was induced in broiler chickens in cages (battery system). The oocysts were collected from already infected poultry guts and were processed and sporulated in potassium dichromate solution according to the standard method of floatation and then the sporulated oocysts were counted by McMaster technique and were inoculated into birds as per required dose rate to induce coccidiosis in birds of group A, B, C and D.
Coccidiosis was diagnosed on standard postmortem procedure and oocyst counting of the fecal droppings of the infected birds. Group A, B and C was medicated by sulphachlorpyarazine (Esb3), steroidal sapogenin (cocciguard) and amprolium (cocoban) respectively. Group D were infected but left un-medicated positive control and group E were left non infected and non medicated negative control.
The therapeutic efficacy of the above mentioned drugs were evaluated by oocyst counting in droppings of all the infected treated groups and by intestinal lesion scoring, bloody diarrhea. Morbidity and mortality were recorded in all infected groups before and after treatment. Oocyst counting was done in all groups after induction of coccidial infection and at the end of the treatment in all medicated groups.
Oocyst counting and intestinal lesion scoring were helpful in evaluation of the therapeutic efficacy of the cocciguard (steroidal sapogenin) in comparison with the chemical anticoccidial drugs.
The following recorded parameters, oocyst counting, morbidity, mortality, bloody diarrhea, intestinal lesion scoring were subjected to statistical analysis using one way analysis of variance, to detect the therapeutic efficacy difference between treatments and the results obtained are as follows.
Oocyst per gram was 1.49*0.78, 20.36±1.89, 2.66±1.47 and 47.7±4.3 for suiphachlorpyrazine, steroidal sapogenin, amprolium and positive control respectively.
Morbidity recorded was 1.5±0.87, 2.75±0.47, 2.25±0.25, and 2.25±0.25 for sulphachlorpyrazine, steroidal sapogenin amprolium and positive control respectively.
Mortality recorded was 0.40±0.24, 2.40±0.24, 1.80±0.2, and 2.40±0.24 for suiphachiorpyrazine, steroidal sapogenin, amprolium and positive control.
Intestinal lesion scoring recorded was 1.5±0.35, 2.5±0.20, and 1.62±0.12 for sulphachlorpyrazine, steroidal sapogenin, and amprolium respectively.
Survival rate recorded was 56%, 20%, 33.30%, 0%, and 93.3% in suiphachlorpyrazine, steroidal sapogenin, amprolium, positive control, and negative control group respectively.
Percent reduction in oocyst per gram was recorded 97.48%, 18.58%, and 97.19% in suiphachiorpyrazine, steroidal sapogenin and amprolium respectively.
Availability: Items available for loan: UVAS Library [Call number: 1016,T] (1).
11.
In Vitro On The Antibacterial Potential Of Ocimum Basilicum (Sweet Basil) And Glycyrrhiza Glabra (Licorice)
by Humaira Tahir | Prof.Dr.Muhammad Ashraf | Dr.Jawaria Ali | Dr.Muhammad Ovais Omer.
Material type: ; Format:
print
; Nature of contents: ; Literary form: Publisher: 2008Dissertation note: This study was designed to test the antibacterial activity of Ocimum basilicum L. (Sweet basil) and Glycyrrhiza glabra (Licorice) extracts in four different solvents i.e. petroleum ether, chloroform, methanol and water against Staphylococcus aureus, Bacillus subtilis, Escherichia co/i and Salmonella typhi. Glycyrrhiza glabra roots were cut into small pieces and Ocimum basilicum L leaves were collected, washed, air dried, grounded and dried in desiccators. Each plant material was weighed and 20.0 grams of it was taken in four different bottles.500.0 ml of each solvent was added in the respective bottle. Plant material was macerated for three days. The extracts were filtered by whatmann's filter paper, dried in vacuum desiccators and the powder mass obtained was weighed and then reconstituted in respective solvent to get the final extract of known concentrations.Each of the bacteria was inoculated separately in the Nutrient Agar medium in a concentration of 1 CFU/ml and the media was poured in petri dishes and was allowed to solidify.Six wells of 1 .Ocentimeter diameter were cut in each plate with the help of sterile cork borer. In three wells equal volume of known concentrations of plant extracts was poured with the help of micropipettes. In 4th well, penicillin G (l600ig per well) and in the 5th well Gentamicin (l000ig per well) was taken as a positive control. In the 6th well, respective solvent was taken as a negative control. The plates were incubated at 37 degree Celsius for 24 hours and the DIZ was calculated in millimeters. Each experiment was performed in five replicates .Both plant extracts showed considerable activity against gram positive and gram negative bacteria. Ocimum basilicum non-polar components had good activity against gram positive than gram negative bacteria while Glycyrrhiza glabra methanol extract showed greater activity against all tested bacteria.
Availability: Items available for loan: UVAS Library [Call number: 1035,T] (1).
12.
Evaluation Of Antibacterial Combination Therapy Against Field Isolates Of Various Bacteria
by Sadia Arshad Rana | Dr.Muhammad Ovais Omer | Dr.Jawaria Ali | Prof.Dr.Muhammad Ashraf.
Material type: ; Format:
print
Publisher: 2008Dissertation note: This study was designed to find out the susceptibility of various selected antibacterial combinations (Colistin and Tylosine; Lincomycin and Spectinomycin; Amoxycillin and Cloxacillin) against field isolates of Gram positive (Staphiococcus spp. and Streptococcus spp.) and Gram negative (Salmonella and E.coli) pathogens in vitro from poultry. All the used antibacterials were in salt form so were soluble in water for injection. Bacterial pathogens were dollected from field source and were transported to laboratory where those were isolated, identified and characterized by slide method and biochemical teclmiqiies. Bacterial activity_was determined by calculating the Minimum Inhibitory Concentrations (MIC) of all the antibacterials alone as well as in combination in their respective groups by tube dilutin method. The lowest concentration (highest dilution) of antibiotic alone or in combination, preventing appearance of turbidity is considered as to be the MIC. Results were computed statistically and analyzed.
Colistin and Ty!Qsin did not exhibit significant results against all selected bacterial isolates although showed efficacy against Gram negative and gram positive isolates respectively. Lincomycin and Spectinomycin attributed considerable activity in combination as compared to the alone agent against almost all isolates. Amoxycillin and Cloxacillin in combination exerted significant results against Gram positive bacteria than alone as compared to Gram negative pathogens.
Availability: Items available for loan: UVAS Library [Call number: 1043,T] (1).
13.
Effect Of Two-Stroke Auto Rickshaw Smoke Solution On Wound Healing In Mice
by Aqeela Iqbal | Prof.Dr.Muhammad Ashraf | Dr.Muhammad Ovais Omer | Prof.Dr.Zafar.
Material type: ; Format:
print
Publisher: 2009Dissertation note: The use of 3-wheel auto-rickshaws powered by a 2-stroke engine is widespread in south Asia; exhaust from these vehicles may cause different types of toxicities resulting in different pathologies. The aim of this study was to explore the association between exposure to 2-stroke auto-rickshaw smoke solution (2SARSS) and wound healing. The in vivo model of wound healing was customized to evaluate different stages of wound healing. A total of 56 mice were used of aged 4-5 weeks. After anesthetizing and hair removing from dorsum side of mice a wound of 3mm diameter was generated with the help of a sterile wound puncture. Smoke was collected in the lOOml distilled water from six 2-stroke rickshaws and then these solutions were filtered by syringe filters. 10% ointment of all the samples was prepared in Petroleum Jelly and applied to the experimental group, petroleum jelly to positive control group, no treatment was applied to negative control. Macroscopic evaluation was done by using high resolution camera photographs and measurement of wound by vernier caliper on alternate days. At the end of the study (day 12) histological evaluation was done when the control group wounds were healed. Histological examination revealed a significant delay in the outcome of re-epithelialization, dermal matrix regeneration, and maturation of collagen bundles among all 2SARSS-exposed wounds. Delayed activation of neovascularization was seen in the 2SARSS-treated groups at day 12 post-wounding. The Abbot curve, angular spectrum, and several other 3D surface parameters of reverse wound topographies revealed a highly significant reduction (p<O.OOl) in angiogenesis. These results demonstrate that application of 2SARSS causes a substantial delay in the progression of angiogenesis, resulting in delayed onset of wound healing. These observations validate the damaging effects of 2SARSS on wound healing. Thus, people who are directly or indirectly exposed to this toxic exhaust are expected to have delayed wound healing, which could result in chronic wounds.
Availability: Items available for loan: UVAS Library [Call number: 1083,T] (1).
14.
Designing A Lead (Drug)Molecule To Block The Dna Binding Site Of Cancer-Causing E2F3 Transcription Factor
by Muzammal Hussain | Dr.Aqeel Javeed | Dr.Muhammad | Prof.Dr.Muhammad Ashraf.
Material type: ; Format:
print
Publisher: 2009Dissertation note: As transcriptional factors are the current area of concern in novel anticancer drug designing, this study was designed to develop a suitable drug (lead) molecule to block the DNA-binding site of cancer-causing E2F3 transcription factor (overexpressed in prostate, lung, bladder cancers) by using computer-aided drug design approach and implementing homology modeling, molecular docking and virtual high-throughput screening techniques. A reasonable 3-dimensional structure model of E2F3 transcription factor was generated by following homology modeling technique and using SWISS-MODEL server. The stereochemical evaluation of the generated model was carried out by using the program PROCHECK. The active site residues of the DNA-binding domain that make critical contacts within the major groove of DNA were determined by analyzing the crystal structure of the template (E2F4). Then, by using this structure model a chemical database (containing 3D structures of available chemical compounds) ZINC was virtually screened: only those molecules having molecular weight between 300 to 350, neutral charge, hydrogen bond donors 0/1, hydrogen bond acceptors 3/5, rotatable bonds 2/7 and a value of xLogP between -2 and 4, were taken into account. The compounds yielded by this database filtration step were then subjected to 10 run docking studies with the program AutoDock 4.02 to search for the suitable hits. This step resulted in 31 hits. From
these hits the compounds with binding energy lower than -3.5 Kcal/mol and showing maximum hydrogen bonding interaction with active site were further selected. This step returned 6 compounds which were further evaluated by giving 30 runs of docking in the sense to improve the interaction with the active site residues (hydrogen bonding) and binding energy. 3 compounds with binding energy less than - 4.0 Kcal/mol were further subjected to visual inspection in order to evaluate their binding poses at the active site. One was eliminated and the remaining two were further subject to 50 docking runs see any improvement in ener4gy. One of the them showed a little improvement in biding energy, however, both were suggested as suitable ,leads, as the difference in their binding energies was very small and both were making equal number of hydrogen bonds with the DNA binding site of target F2F3
Availability: Items available for loan: UVAS Library [Call number: 1100,T] (1).
15.
Comparative Efficacy Of Various Antiviral Agents Against Newcastle Disease Virus
by Shahzada Khurram Syed | Dr.Muhammad Ovais Omer | Dr.Aftab | Prof.Dr.Muhammad Ashraf.
Material type: ; Format:
print
; Nature of contents: ; Literary form: Publisher: 2010Dissertation note: In this project, 120 embryonated broiler chicken eggs of 9th days were obtained from Hi-tech laboratories Pvt Ltd and were placed in WTO quality control laboratory. Eggs were candled for separation of live embryos. For each drug to be tested, embryonated eggs were divided in 8 groups and marked them with lead pencil.
Eggs were disinfected with 70% ethanol. The New castle disease was virus obtained from Department of Microbiology, University of Veterinary and Animal Sciences, Lahore. Virus was identified by haemagglutination inhibition test. To prepare inoculums, stored virus was admixed at room temperature with antibiotics. Each embryonated eggs were inoculated with 0.1 ml of inoculum with the lml of BD syringes.
Eggs were incubated for 72 hours with frequent candling after every 24 hours. After, 72 hours of post inoculation, all live and dead embryos in eggs were kept in refrigerator at 4°C to 8°C. After, 72 hours antiviral groups were checked for the replication of ND virus by means of spot haemagglutination test. Drug's toxicity was checked for the viability of embryo by candling.
Three different concentrations of plant extracts of garlic, glycyrriza and ribavirin in normal saline were evaluated for' their antiviral activity and toxicity in embryonated eggs. Three concentrations for glycyrrhiza 1 5mg/iOOml, 30mg/i OOml and 60mg/i OOml were used. l5mg/lOOmi was neither toxic nor against virus. 6Omg/lOOmi was toxic and killed the embryos. Only 3Omg/lOOmi was antiviral as well as non-toxic.
Three different of concentrations of garlic were lgm/lOOmi, 5gm/lOOmi and 10gm/lOOm!. lgm/lOOmi was not having antiviral activity and it did not stop virus replication. 5gm/lOOmi and lOgm/lOOmi stopped virus replication and also non-toxic.
Three different concentrations 5ig/m1, lOtg/ml, 2Oig/ml of ribavirin were used. The first concentration was not having toxicity and antiviral activity. Second concentration was antiviral as well as non-toxic. Third concentration was toxic and killed the embryos.
This project was designed to investigate the efficacy of ribavirin and plant extracts (garlic and glycyrrhiza) against new castle disease virus and to reduce the cost of antiviral medicines and to explore flora of Pakistan.
Availability: Items available for loan: UVAS Library [Call number: 1104,T] (1).
16.
Chemical Equivalence Of Different Brands Of Amoxicillin Trihydrate And Its Minimum Inhibitory Concentration
by Rana Adnan Ali | Prof.Dr.Muhammad Ashraf | Dr aftab Ahmad | Dr.Muhammad Adil Rasheed.
Material type: ; Format:
print
Publisher: 2011Dissertation note: This project was designed to study the chemical equivalence of different brands of amoxicillin trihydrate (long acting and short acting) approved by the ministry of health and available in the market for veterinary use. Amoxicillin was measured by HPLC method developed and standardized in the laboratory. Limit of detection (LOD) and limit of quantification (LOQ) of the amoxicillin trihydrate was determined. Solutions of different concentrations were prepared from amoxicillin trihydrate reference standard for the determination of LOD. and were protected from light and stored at 2-8 oC until used. The LOD calculated by us was 0.100 (µg / ml) and LOQ was 0.5 (µg / ml). Correlation Coefficient should be ? 0.99 and the result obtained by the data was 0.99984050. Chemical equivalence of all brands was determined by using HPLC systems (Shimadzu & Agilent). Concentrations for reference standard (50, 25 and 10 ?g /ml ) and for each brand (Alomox LA, Amovet LA, Farmox LA, Novamox LA, Trioxyl LA, Amoxi-vet, Colimox, and Colimoxin) were used. All the results obtained showed that maximum percentage of assay obtained among long acting was of the brand Farmox LA (101 %) and in case of short acting was of Amoxi-vet (101%). Minimum percentage of assay among long acting was of brand Amovet LA (92 %) and in case of short acting was of Colimox (96%). MIC of amoxicillin against E.coli and Staphylococcus was determined by micro broth dilution test. According to our results 73.33 % E.coli were susceptible and 26.67% were resistant to the amoxicillin trihydrate. Our results showed that 86.67% Staphylococcus were susceptible and 13.33% were resistant to Amoxicillin Trihydrate (Reference Standard). It showed that this antibiotic is still very effective against the diseases produced by the Escherichia.coli and Staphylococcus aureus.
Availability: Items available for loan: UVAS Library [Call number: 1249,T] (1).
17.
Effect Of Diclofenac Sodium On Angiogenesis Using Chorio Allantoic Membrance (Cam) Assay
by Iradat hussain | Dr.Muhammad Ovais Omer | Prof.Dr.Habib | Prof.Dr.Muhammad Ashraf.
Material type: ; Format:
print
Publisher: 2010Dissertation note: Angiogenesis, the growth of new capillary blood vessels in the body, has much more importance in healing and reproduction. The body controls angiogenesis as there is a natural balance between growth and inhibitory factors in healthy tissues.
When this balance is disturbed, the result is either too much or too little angiogenesis. Abnormal blood vessel growth, either excessive or insufficient, is now recognized as a "common denominator" underlying many deadly and debilitating conditions, including cancer, skin diseases, age-related blindness, diabetic ulcers, cardiovascular disease, stroke, and many others.
Treating angiogenesis with anti-angiogenic compounds results in safe and effective anti-angiogenic therapies. In current experiment, focus was on the effect of Diclofenac sodium on angiogenesis using chicken chorio-allantoic membrane (CAM) assay. Fresh fertilized eggs were taken, sprayed with 70% ethanol and incubated at 37 °C (humidity 55-60%). At day 5 of incubation a small window was made on each egg, 4-5 ml of albumin was aspirated, windows were sealed with sterile Para-film and eggs were returned to incubator. Concentrations i.e. 0.7%, 0.5%, 0.3% were prepared in distilled water. Filtered concentrations of the Diclofenac sodium were used for experimental analysis and applied to the CAMs on day 7 of incubation. Macroscopic vascular changes were evidently observed among all treated CAMs on day 6 of incubation. There was reduction in total area and diameter of primary, secondary and tertiary blood vessels was observed after treating CAMs with Diclofenac sodium. By using SPIP (Scanning probe image processor) software, 3D surface roughness measurements, Sa (surface area ), Sq (root mean square), Ssk (surface deviation), Sku (peakedness), Sdr (ratio of increment of interfacial area of a surface over sampling area), Sci (ratio of void volume of the unit sampling area at core zone over root mean square deviation ), Sy (lowest valley), Sz (average absolute height), Ssc (arithmetic mean submit), Sdq (root mean square sloop), Spk (reduce summit height), Sku (kurtosis of surface) , Stdi (texture index), Sk (core roughness depth) were carried out which clearly elaborated anti-angiogenic effect of Diclofenac sodium on CAMs. Diclofenac sodium inhibits angiogenesis, which is a common denominator shared by various major disease.
Availability: Items available for loan: UVAS Library [Call number: 1250,T] (1).
18.
Immunomodulatory Effect Of Renessans In Laboratory Animals
by Khurram Aamir | Prof. Dr.Muhammad Ashraf | Dr. Aqeel Javeed | Malik Allah.
Material type: ; Format:
print
; Literary form:
drama
Publisher: 2013Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 1759,T] (1).
19.
Evaluation Of Imunomodulatory Effect Of Naproxen In Mice
by Farah javaid | Dr.Aqeel jaaveed | Prof. Dr.Muhammad Ashraf.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 2013Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 1848,T] (1).
20.
Toxicological Evaluation Of Cetrimimide Chlorhexidin And Thiomersal Alone And In Combination
by Toseef Fatima Noshahi | Prof. Dr. Muhammad Ashraf | Dr.Imran Altaf | Dr.Muhammad Adil Rasheed.
Material type: ; Format:
print
Publisher: 2014Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 1880,T] (1).
21.
Evaluation Of Antiviral And Cytotoxic Activity Of Calotropis Procera Against Foot And Mouth Disease Virus
by Uzma saher | Dr. Aqeel javeed | Prof. Dr.Muhammad Ashraf.
Material type: ; Format:
print
Publisher: 2014Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 1891,T] (1).
